PENTASQUAREIn the
Pentasquare slide with counting grid, the microscopic counting of the cells present in the urine sediment, is based on the same principle of the glass counting chambers actually present on the market
(Bürker, Thoma-Zeiss, Neubauer).
The above mentioned counting chambers determine, through appropriate calculations, the number of the elements per ml, present in a sample of urine.
The surface on which the sample is spread in the chamber is divided in spaces defined by a grid.
Inside the squares, the volume of the samples is predetermined and consequently, through a calculation procedure, there is a direct connection between the number of cells counted per square and the
whole amount in the urine sample under examination.
Pentasquare slide is different from the traditional glass counting chamber for its easiness in the use.
It helps the operator in the daily routine and decreases drastically some possible causes of error with the use of such chambers.
In the
Pentasquare slide the volume of the samples limited by the grid is pre-determined and constant in all the cells (every slide is subject to strict quality controls during the production).
The area delimited by the grid is 3 mm by 3 mm divided in 5 squares with a side of 1 mm.
Every square of 1mm side is also divided in 9 small squares with a side of 0,333 mm.
Every grid is divided in 45 small squares with a side of 0,333 mm.
Capacities0,5 ul - the whole counting grid
0,1 ul - each of the 5 squares of 1x1mm side
0,0111 ul - each of the 9 small squares of
0,333 x 0,333 mm side